@article{lehmann_optical_2020,

title = {Optical Tweezers Approaches for Probing Multiscale Protein Mechanics and Assembly},

author = {K Lehmann and M Shayegan and Gerhard A. Blab and N R Forde},

url = {https://www.frontiersin.org/articles/10.3389/fmolb.2020.577314/full},

doi = {10.3389/fmolb.2020.577314},

year  = {2020},

date = {2020-01-01},

urldate = {2020-01-01},

journal = {Frontiers in Molecular Biosciences},

volume = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mohammadian_integrated_2020,

title = {Integrated super resolution fluorescence microscopy and transmission electron microscopy},

author = {Sajjad Mohammadian and Alexandra V Agronskaia and Gerhard A. Blab and E G van Donselaar and C de Heus and N Liv and J Klumperman and Hans C. Gerritsen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085254573&doi=10.1016%2fj.ultramic.2020.113007&partnerID=40&md5=d0486d73138b228cf6091bd2e59de054},

doi = {10.1016/j.ultramic.2020.113007},

year  = {2020},

date = {2020-01-01},

journal = {Ultramicroscopy},

volume = {215},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{soeteman-hernandez_challenges_2020,

title = {Challenges of implementing nano-specific safety and safe-by-design principles in academia},

author = {L G Soeteman-Hernández and Gerhard A. Blab and A Carattino and F Dekker and S Dekkers and M van der Linden and A van Silfhout and C W Noorlander},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089135360&doi=10.1016%2fj.impact.2020.100243&partnerID=40&md5=95e711c68da63f49c51e21c9a9b22648},

doi = {10.1016/j.impact.2020.100243},

year  = {2020},

date = {2020-01-01},

journal = {NanoImpact},

volume = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{van_hest_towards_2019,

title = {Towards robust and versatile single nanoparticle fiducial markers for correlative light and electron microscopy},

author = { Jacobine J.H.A. van Hest and Alexandra V. Agronskaia and Jantina Fokkema and F. Montanarella and A. Gregorio Puig and Celso de Mello Donega and Andries Meijerink and Gerhard A. Blab and Hans C. Gerritsen},

doi = {10.1111/jmi.12778},

year  = {2019},

date = {2019-01-01},

journal = {Journal of Microscopy},

volume = {274},

number = {1},

pages = {13--22},

abstract = {Fiducial markers are used in correlated light and electron microscopy (CLEM) to enable accurate overlaying of fluorescence and electron microscopy images. Currently used fiducial markers, e.g. dye-labelled nanoparticles and quantum dots, suffer from irreversible quenching of the luminescence after electron beam exposure. This limits their use in CLEM, since samples have to be studied with light microscopy before the sample can be studied with electron microscopy. Robust fiducial markers, i.e. luminescent labels that can (partially) withstand electron bombardment, are interesting because of the recent development of integrated CLEM microscopes. In addition, nonintegrated CLEM setups may benefit from such fiducial markers. Such markers would allow switching back from EM to LM and are not available yet. Here, we investigate the robustness of various luminescent nanoparticles (NPs) that have good contrast in electron microscopy; 130 nm gold-core rhodamine B-labelled silica particles, 15 nm CdSe/CdS/ZnS core–shell–shell quantum dots (QDs) and 230 nm Y 2 O 3 :Eu 3+ particles. Robustness is studied by measuring the luminescence of (single) NPs after various cycles of electron beam exposure. The gold-core rhodamine B-labelled silica NPs and QDs are quenched after a single exposure to 60 ke −  nm –2 with an energy of 120 keV, while Y 2 O 3 :Eu 3+ NPs are robust and still show luminescence after five doses of 60 ke − nm –2 . In addition, the luminescence intensity of Y 2 O 3 :Eu 3+ NPs is investigated as function of electron dose for various electron fluxes. The luminescence intensity initially drops to a constant value well above the single particle detection limit. The intensity loss does not depend on the electron flux, but on the total electron dose. The results indicate that Y 2 O 3 :Eu 3+ NPs are promising as robust fiducial marker in CLEM. Lay Description: Luminescent particles are used as fiducial markers in correlative light and electron microscopy (CLEM) to enable accurate overlaying of fluorescence and electron microscopy images. The currently used fiducial markers, e.g. dyes and quantum dots, loose their luminescence after exposure to the electron beam of the electron microscope. This limits their use in CLEM, since samples have to be studied with light microscopy before the sample can be studied with electron microscopy. Robust fiducial markers, i.e. luminescent labels that can withstand electron exposure, are interesting because of recent developments in integrated CLEM microscopes. Also nonintegrated CLEM setups may benefit from such fiducial markers. Such markers would allow for switching back to fluorescence imaging after the recording of electron microscopy imaging and are not available yet. Here, we investigate the robustness of various luminescent nanoparticles (NPs) that have good contrast in electron microscopy; dye-labelled silica particles, quantum dots and lanthanide-doped inorganic particles. Robustness is studied by measuring the luminescence of (single) NPs after various cycles of electron beam exposure. The dye-labelled silica NPs and QDs are quenched after a single exposure to 60 ke − nm –2 with an energy of 120 keV, while lanthanide-doped inorganic NPs are robust and still show luminescence after five doses of 60 ke − nm –2 . In addition, the luminescence intensity of lanthanide-doped inorganic NPs is investigated as function of electron dose for various electron fluxes. The luminescence intensity initially drops to a constant value well above the single particle detection limit. The intensity loss does not depend on the electron flux, but on the total electron dose. The results indicate that lanthanide-doped NPs are promising as robust fiducial marker in CLEM. © 2019 The Authors. Journal of Microscopy published by JohnWiley & Sons Ltd on behalf of Royal Microscopical Society.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mohammadian_high_2019,

title = {High accuracy, fiducial marker-based image registration of correlative microscopy images},

author = {Sajjad Mohammadian and Jantina Fokkema and Alexandra V Agronskaia and N Liv and C de Heus and E van Donselaar and Gerhard A. Blab and J Klumperman and Hans C Gerritsen},

doi = {10.1038/s41598-019-40098-4},

year  = {2019},

date = {2019-01-01},

journal = {Scientific Reports},

volume = {9},

number = {1},

abstract = {Fluorescence microscopy (FM) and electron microscopy (EM) are complementary techniques. FM affords examination of large fields of view and identifying regions of interest but has a low resolution. EM exhibits excellent resolution over a limited field of view. The combination of these two techniques, correlative microscopy, received considerable interest in the past years and has proven its potential in biology and material science. Accurate correlation of FM and EM images is, however, challenging due to the differences in contrast mechanism, size of field of view and resolution. We report an accurate, fast and robust method to correlate FM and EM images using low densities of fiducial markers. Here, 120 nm diameter fiducial markers consisting of fluorescently labelled silica coated gold nanoparticles are used. The method relies on recording FM, low magnification EM and high magnification EM images. Two linear transformation matrices are constructed, FM to low magnification EM and low magnification EM to high magnification EM. Combination of these matrices results in a high accuracy transformation of FM to high magnification EM coordinates. The method was tested using two different transmission electron microscopes and different Tokuyasu and Lowicryl sections. The overall accuracy of the correlation method is high, 5–30 nm. © 2019, The Author(s).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Fokkema2018,

title = {Fluorescently Labelled Silica Coated Gold Nanoparticles as Fiducial Markers for Correlative Light and Electron Microscopy},

author = {Jantina Fokkema and Job Fermie and Nalan Liv and Dave J. van den Heuvel and Tom O. M. Konings and Gerhard A. Blab and Andries Meijerink and Judith Klumperman and Hans C. Gerritsen},

url = {https://doi.org/10.1038/s41598-018-31836-1},

doi = {10.1038/s41598-018-31836-1},

year  = {2018},

date = {2018-09-11},

journal = {Scientific Reports},

volume = {8},

number = {1},

pages = {13625},

abstract = {In this work, gold nanoparticles coated with a fluorescently labelled (rhodamine B) silica shell are presented as fiducial markers for correlative light and electron microscopy (CLEM). The synthesis of the particles is optimized to obtain homogeneous, spherical core-shell particles of arbitrary size. Next, particles labelled with different fluorophore densities are characterized to determine under which conditions bright and (photo)stable particles can be obtained. 2 and 3D CLEM examples are presented where optimized particles are used for correlation. In the 2D example, fiducials are added to a cryosection of cells whereas in the 3D example cells are imaged after endocytosis of the fiducials. Both examples demonstrate that the particles are clearly visible in both modalities and can be used for correlation. Additionally, the recognizable core-shell structure of the fiducials proves to be very powerful in electron microscopy: it makes it possible to irrefutably identify the particles and makes it easy to accurately determine the center of the fiducials.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{van_hest_role_2018,

title = {The Role of a Phonon Bottleneck in Relaxation Processes for Ln-Doped NaYF4 Nanocrystals},

author = { Jacobine J. H. A. van Hest and Gerhard A. Blab and Hans C. Gerritsen and Celso de Mello Donega and Andries Meijerink},

url = {https://doi.org/10.1021/acs.jpcc.7b11171},

doi = {10.1021/acs.jpcc.7b11171},

issn = {1932-7447},

year  = {2018},

date = {2018-01-01},

urldate = {2018-09-11},

journal = {The Journal of Physical Chemistry C},

volume = {122},

number = {7},

pages = {3985--3993},

abstract = {The localized inner 4f shell transitions of lanthanide ions are largely independent of the local surroundings. The luminescence properties of Ln3+ ions doped into nanocrystals (NCs) are therefore similar to those in bulk crystals. Quantum size effects, responsible for the unique size-dependent luminescence of semiconductor NCs, are generally assumed not to influence the optical properties of Ln3+-doped insulator NCs. However, phonon confinement effects have been reported to hamper relaxation between closely spaced Stark levels in Ln3+-doped NCs. At cryogenic temperatures emission and excitation from higher Stark levels was observed for Ln3+ ions in NCs only and were explained by a cutoff in the acoustic phonon spectrum. Relaxation would be inhibited as no resonant low energy (long wavelength) acoustic phonon modes can exist in nanometer sized crystals, and this prevents relaxation by direct phonon emission between closely spaced Stark levels. This phenomenon is known as a phonon bottleneck. Here, we investigate the role of phonon confinement in Ln-doped NCs. High resolution emission spectra at temperatures down to 2.2 K are reported for various Ln3+ ions (Er3+, Yb3+, Eu3+) doped into monodisperse 10 nm NaYF4 NCs and compared with spectra for bulk (microcrystalline) material. Contrary to previous reports, we find no evidence for phonon bottleneck effects in the emission spectra. Emission from closely spaced higher Stark levels is observed only at high excitation powers and is explained by laser heating. The present results indicate that previously reported effects in NCs may not be caused by phonon confinement.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{doi:10.1021/acs.jpcc.7b06549,

title = {Probing the Influence of Disorder on Lanthanide Luminescence Using Eu-Doped LaPO4 Nanoparticles},

author = { Jacobine J.H.A. van Hest and Gerhard A. Blab and Hans C. Gerritsen and Celso de Mello-Donega and Andries Meijerink},

url = {http://dx.doi.org/10.1021/acs.jpcc.7b06549},

doi = {10.1021/acs.jpcc.7b06549},

year  = {2017},

date = {2017-01-01},

journal = {The Journal of Physical Chemistry C},

volume = {121},

number = {35},

pages = {19373-19382},

abstract = {Lanthanide-doped nanocrystals (NCs) differ from their bulk counterparts due to their large surface to volume ratio. It is generally assumed that the optical properties are not affected by size effects as electronic transitions occur within the well-shielded 4f shell of the lanthanide dopant ions. However, defects and disorder in the surface layer can affect the luminescence properties. Trivalent europium is a suitable ion to investigate the subtle influence of the surface, because of its characteristic luminescence and high sensitivity to the local environment. Here, we investigate the influence of disorder in NCs on the optical properties of lanthanide dopants by studying the inhomogeneous linewidth, emission intensity ratios, and luminescence decay curves for LaPO4:Eu3+ samples of different sizes (4 nm to bulk) and core–shell configurations (core, core–isocrystalline shell, and core–silica shell). We show that the emission linewidths increase strongly for NCs. The ratio of the intensities of the forced electric dipole (ED) and magnetic dipole (MD) transitions, a measure for the local symmetry distortion around Eu3+ ions, is higher for samples with a large fraction of Eu3+ ions close to the surface. Finally, we present luminescence decay curves revealing an increased nonradiative decay rate for Eu3+ in NCs. The effects are strongest in core and core–silica shell NCs and can be reduced by growth of an isocrystalline LaPO4 shell. The present systematic study provides quantitative insight into the role of surface disorder on the optical properties of lanthanide-doped NCs. These insights are important in emerging applications of lanthanide-doped nanocrystals.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{van_hest_incorporation_2016,

title = {Incorporation of Ln-Doped LaPO4 Nanocrystals as Luminescent Markers in Silica Nanoparticles},

author = {Jacobine J.H.A. van Hest and Gerhard A. Blab and Hans C. Gerritsen and Celso de Mello-Donega and Andries Meijerink},

url = {http://nanoscalereslett.springeropen.com/articles/10.1186/s11671-016-1465-y},

doi = {10.1186/s11671-016-1465-y},

issn = {1931-7573, 1556-276X},

year  = {2016},

date = {2016-01-01},

urldate = {2016-07-11},

journal = {Nanoscale Research Letters},

volume = {11},

number = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Zuckermann2015,

title = {Motor properties from persistence: a linear molecular walker lacking spatial and temporal asymmetry},

author = {Martin J. Zuckermann and Christopher N. Angstmann and Regina Schmitt and Gerhard A. Blab and Elizabeth H.C. Bromley and Nancy R. Forde and Heiner Linke and Paul M.G. Curmi},

url = {http://iopscience.iop.org/1367-2630/17/5/055017/},

doi = {10.1088/1367-2630/17/5/055017},

year  = {2015},

date = {2015-05-05},

journal = {New Journal of Phyiscs},

volume = {17},

pages = {055017},

abstract = {The stepping direction of linear molecular motors is usually defined by a spatial asymmetry of the motor, its track, or both. Here we present a model for a molecular walker that undergoes biased directional motion along a symmetric track in the presence of a temporally symmetric chemical cycle. Instead of using asymmetry, directionality is achieved by persistence. At small load force the walker can take on average thousands of steps in a given direction until it stochastically reverses direction. We discuss a specific experimental implementation of a synthetic motor based on this design and find, using Langevin and Monte Carlo simulations, that a realistic walker can work against load forces on the order of picoNewtons with an efficiency of ~18%, comparable to that of kinesin. In principle, the walker can be turned into a permanent motor by externally monitoring the walker's momentary direction of motion, and using feedback to adjust the direction of a load force. We calculate the thermodynamic cost of using feedback to enhance motor performance in terms of the Shannon entropy, and find that it reduces the efficiency of a realistic motor only marginally. We discuss the implications for natural protein motor performance in the context of the strong performance of this design based only on a thermal ratchet.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{vanImhof2015,

title = {Jammed elastic shells – a 3D experimental soft frictionless granular system },

author = {Jissy Jose and Gerhard A. Blab and Alfons van Blaaderen and Arnout Imhof},

url = {http://pubs.rsc.org/en/content/articlelanding/2015/sm/c4sm02098g},

doi = {10.1039/C4SM02098G },

year  = {2015},

date = {2015-03-07},

journal = {Soft Matter},

volume = {11},

number = {9},

pages = {1800-1813},

abstract = {We present a new experimental system of monodisperse, soft, frictionless, fluorescent labeled elastic shells for the characterization of structure, universal scaling laws and force networks in 3D jammed matter. The elastic shells in a jammed packing are deformed in such a way that at each contact one of the shells buckles with a dimple and the other remain spherical, closely resembling overlapping spheres. Using confocal microscopy, we obtained 3D stacks of images of shells at different volume fractions which were subsequently processed in ImageJ software to find their coordinates. The determination of 3D coordinates involved three steps: locating the edges of shells in all 2D slices, analyzing their shape and subsequently finding their 2D coordinates, and finally determining their 3D centers by grouping the corresponding 2D coordinates. From this analysis routine we obtained particle coordinates with sub-pixel accuracy. In a contact pair we also identified the shell that underwent buckling forming a dimple by analyzing the intensity profile of a line that connects the centers of particle pairs. The amorphous structure of the packing was analyzed as a function of distance to the jamming threshold by investigating the radial distribution function, bond order parameters, contact numbers and the number of dimples per particle (buckling number), which is a unique property of this system. We find that the power law scaling of the contact number with excess volume fraction deviated from theoretical and computer simulation predictions. In addition, the buckling number also showed a similar scaling as that of the contact number with distance to the jamming transition.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{PIP:PIP2702,

title = {3D-printed external light trap for solar cells},

author = {Lourens van Dijk and Ulrich W. Paetzold and Gerhard A. Blab and Ruud E.I. Schropp and Marcel di Vece},

url = {http://dx.doi.org/10.1002/pip.2702},

doi = {10.1002/pip.2702},

issn = {1099-159X},

year  = {2015},

date = {2015-01-01},

journal = {Progress in Photovoltaics: Research and Applications},

pages = {n/a--n/a},

abstract = {We present a universally applicable 3D-printed external light trap for enhanced absorption in solar cells. The macroscopic external light trap is placed at the sun-facing surface of the solar cell and retro-reflects the light that would otherwise escape. The light trap consists of a reflective parabolic concentrator placed on top of a reflective cage. Upon placement of the light trap, an improvement of 15% of both the photocurrent and the power conversion efficiency in a thin-film nanocrystalline silicon (nc-Si:H) solar cell is measured. The trapped light traverses the solar cell several times within the reflective cage thereby increasing the total absorption in the cell. Consequently, the trap reduces optical losses and enhances the absorption over the entire spectrum. The components of the light trap are 3D printed and made of smoothened, silver-coated thermoplastic. In contrast to conventional light trapping methods, external light trapping leaves the material quality and the electrical properties of the solar cell unaffected. To explain the theoretical operation of the external light trap, we introduce a model that predicts the absorption enhancement in the solar cell by the external light trap. The corresponding calculated path length enhancement shows good agreement with the empirically derived value from the opto-electrical data of the solar cell. Moreover, we analyze the influence of the angle of incidence on the parasitic absorptance to obtain full understanding of the trap performance.Copyright © 2015 © 2015 The Authors. Progress in Photovoltaics: Research and Applications published by John Wiley & Sons Ltd.},

note = {pip.2702},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Revalee2014705,

title = {Tethered particle motion reveals that LacI·DNA loops coexist with a competitor-resistant but apparently unlooped conformation},

author = { Joel D. Revalee and Gerhard A. Blab and Henry D. Wilson and Jason D. Kahn and Jens Christian Meiners},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84893515191&partnerID=40&md5=b469b5118033e09f75eb18a2f5dee398},

doi = {10.1016/j.bpj.2013.12.024},

year  = {2014},

date = {2014-02-04},

journal = {Biophysical Journal},

volume = {106},

number = {3},

pages = {705-715},

abstract = {The lac repressor protein (LacI) efficiently represses transcription of the lac operon in Escherichia coli by binding to two distant operator sites on the bacterial DNA and causing the intervening DNA to form a loop. We employed single-molecule tethered particle motion to observe LacI-mediated loop formation and breakdown in DNA constructs that incorporate optimized operator binding sites and intrinsic curvature favorable to loop formation. Previous bulk competition assays indirectly measured the loop lifetimes in these optimized DNA constructs as being on the order of days; however, we measured these same lifetimes to be on the order of minutes for both looped and unlooped states. In a range of single-molecule DNA competition experiments, we found that the resistance of the LacI-DNA complex to competitive binding is a function of both the operator strength and the interoperator sequence. To explain these findings, we present what we believe to be a new kinetic model of loop formation and DNA competition. In this proposed new model, we hypothesize a new unlooped state in which the unbound DNA-binding domain of the LacI protein interacts nonspecifically with nonoperator DNA adjacent to the operator site at which the second LacI DNA-binding domain is bound. © 2014 Biophysical Society.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2050-6120-2-3-035001,

title = {Phasor based analysis of FRET images recorded using spectrally resolved lifetime imaging},

author = {Farzad Fereidouni and Gerhard A. Blab and Hans C. Gerritsen},

url = {http://stacks.iop.org/2050-6120/2/i=3/a=035001},

year  = {2014},

date = {2014-01-01},

journal = {Methods and Applications in Fluorescence},

volume = {2},

number = {3},

pages = {035001},

abstract = {The combined analysis of spectral and lifetime images has the potential to provide more accurate and more detailed information about Förster resonance energy transfer (FRET). We have developed a novel FRET analysis method to analyze images recorded by multispectral lifetime imaging. The new method is based on a phasor approach and facilitates the simultaneous analysis of decay kinetics of donor and acceptor molecules. The method is applicable to both molecules that exhibit a mono-exponential decay and a bi-exponential decay. As an example we show the possibility of extracting the energy transfer efficiency and the fraction of interacting molecules even in the presence of non-interacting molecules. The reliability of the method is investigated by comparing it with conventional FRET-FLIM analyses. We show that, with the same number of detected photons, the spectrally resolved phasor approach provides higher accuracy than other analysis methods; the confidence interval is improved and the FRET efficiency is closer to the real value.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Knaus2013,

title = {Label-free fluorescence microscopy in fungi},

author = {Helene Knaus and Gerhard A. Blab and G. Jerre van Veluw and Hans C. Gerritsen and Han A.B. Wösten},

url = {http://www.sciencedirect.com/science/article/pii/S1749461313000304},

doi = {http://dx.doi.org/10.1016/j.fbr.2013.05.003},

issn = {1749-4613},

year  = {2013},

date = {2013-01-01},

journal = {Fungal Biology Reviews},

number = {0},

pages = {-},

abstract = {Abstract Label-free fluorescence microscopy detects fluorescence originating from endogenous fluorophores, such as NAD(P)H, melanin and flavins. The emitted fluorescence (spectrum, lifetime and polarization) is characteristic for the molecule and its environment. In most cases, a specimen contains multiple autofluorescent molecules contributing to the overall fluorescence. Methods have been developed to break down the fluorescence into the contribution of its individual components. As a result, label-free microscopy can map biochemical properties of fluorophores spatially and over time at the level of the organism, tissue and cells. This is of interest for fungal cell biology and development. Moreover, it can be used in biotechnological applications to monitor the metabolic state within a bioreactor or to monitor the formation of secondary metabolites. Combining morphological and biochemical properties can also lead to new developments in fungal taxonomy, biomedical diagnostics, as well as the screening of fungal products such as mushrooms.},

note = {in press},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Fereidouni2013,

title = {Blind unmixing of spectrally resolved lifetime images},

author = {Farzad Fereidouni and Gerhard A. Blab and Hans C. Gerritsen},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84892490015&partnerID=40&md5=2a47200af50b1f0806307feacaca9673},

doi = {10.1117/1.JBO.18.8.086006},

year  = {2013},

date = {2013-01-01},

journal = {Journal of Biomedical Optics},

volume = {18},

number = {8},

note = {cited By 6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Knaus20136345,

title = {Monitoring the metabolic state of fungal hyphae and the presence of melanin by nonlinear spectral imaging},

author = {Helene Knaus and Gerhard A. Blab and Alexandra V. Agronskaia and Dave J. van den Heuvel and Hans C. Gerritsen and Han A.B. Wösten},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84885037949&partnerID=40&md5=d7640036730e5e1be25a63231c0c47ec},

doi = {10.1128/AEM.02291-13},

year  = {2013},

date = {2013-01-01},

journal = {Applied and Environmental Microbiology},

volume = {79},

number = {20},

pages = {6345-6350},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kovacic2012,

title = {Design and construction of a one-dimensional DNA track for an artificial molecular motor},

author = {Suzana Kovacic and Laleh Samii and Dek N. Woolfson and Paul M.G. Curmi and Heiner Linke and Nancy R. Forde and Gerhard A. Blab},

url = {http://www.hindawi.com/journals/jnm/2012/109238/},

doi = {10.1155/2012/109238},

issn = {16874110},

year  = {2012},

date = {2012-01-01},

journal = {Journal of Nanomaterials},

volume = {2012},

abstract = {DNA is a versatile heteropolymer that shows great potential as a building block for a diverse array of nanostructures. We present here a solution to the problem of designing and synthesizing a DNA-based nanostructure that will serve as the track along which an artificial molecular motor processes. This one-dimensional DNA track exhibits periodically repeating elements that provide specific binding sites for the molecular motor. Besides these binding elements, additional sequences are necessary to label specific regions within the DNA track and to facilitate track construction. Designing an ideal DNA track sequence presents a particular challenge because of the many variable elements that greatly expand the number of potential sequences from which the ideal sequence must be chosen. In order to find a suitable DNA sequence, we have adapted a genetic algorithm which is well suited for a large but sparse search space. This algorithm readily identifies long DNA sequences that include all the necessary elements to both facilitate DNA track construction and to present appropriate binding sites for the molecular motor. We have successfully experimentally incorporated the sequence identified by the algorithm into a long DNA track meeting the criteria for observation of the molecular motor's activity. © 2012 Suzana Kovacic et al.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Fereidouni2011248,

title = {A modified phasor approach for analyzing time-gated fluorescence lifetime images},

author = {Farzad Fereidouni and Alessandro Esposito and Gerhard A. Blab and Hans C. Gerritsen},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-81255168136&partnerID=40&md5=8c5891d4a795370fea469dc374564281},

year  = {2011},

date = {2011-01-01},

journal = {Journal of Microscopy},

volume = {244},

number = {3},

pages = {248-258},

abstract = {Fluorescence lifetime imaging is a versatile tool that permits mapping the biochemical environment in the cell. Among various fluorescence lifetime imaging techniques, time-correlated single photon counting and time-gating methods have been demonstrated to be very efficient and robust for the imaging of biological specimens. Recently, the phasor representation of lifetime images became popular because it provides an intuitive graphical view of the fluorescence lifetime content of the images and, when used for global analysis, significantly improves the overall S/N of lifetime analysis. Compared to time-correlated single photon counting, time gating methods can provide higher count rates (∼10 MHz) but at the cost of truncating and under sampling the decay curve due to the limited number of gates commonly used. These limitations also complicate the implementation of the phasor analysis for time-gated data. In this work, we propose and validate a theoretical framework that overcomes these problems. This modified approach is tested on both simulated lifetime images and on cells. We demonstrate that this method is able to retrieve two lifetimes from time gating data that cannot be resolved using standard (non-global) fitting techniques. The new approach increases the information that can be obtained from typical measurements and simplifies the analysis of fluorescence lifetime imaging data. © 2011 Utrecht University Journal of Microscopy © 2011 Royal Microscopical Society.},

note = {cited By (since 1996) 4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Farré201121370,

title = {Positional stability of holographic optical traps},

author = {Arnau Farré and Marjan Shayegan and Carol López-Quesada and Gerhard A. Blab and Mario Montes-Usategui and Nancy R. Forde and Estela Martín-Badosa},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80054936600&partnerID=40&md5=ee19273928a3c3caa6cbf489d1cac16f},

doi = {10.1364/OE.19.021370},

year  = {2011},

date = {2011-01-01},

journal = {Optics Express},

volume = {19},

number = {22},

pages = {21370-21384},

note = {cited By 6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Samii2011,

title = {Time-dependent motor properties of multipedal molecular spiders},

author = {Laleh Samii and Gerhard A. Blab and Elizabeth H.C. Bromley and Heiner Linke and Paul M.G. Curmi and Martin J. Zuckermann and Nancy R. Forde},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80053028643&partnerID=40&md5=4cd91381870565242d9375cb20679ce3},

doi = {10.1103/PhysRevE.84.031111},

year  = {2011},

date = {2011-01-01},

journal = {Physical Review E - Statistical, Nonlinear, and Soft Matter Physics},

volume = {84},

number = {3},

note = {cited By 9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Farré2010224,

title = {Stretching single DNA molecules to demonstrate high-force capabilities of holographic optical tweezers},

author = {Arnau Farré and Astrid van der Horst and Gerhard A. Blab and Benjamin P.B. Downing and Nancy R. Forde},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77950880891&partnerID=40&md5=de8070d902b91ff9d044ed2d8a48ac6e},

doi = {10.1002/jbio.200900107},

year  = {2010},

date = {2010-01-01},

journal = {Journal of Biophotonics},

volume = {3},

number = {4},

pages = {224-233},

note = {cited By 18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kuwada2010479,

title = {A classical Master equation approach to modeling an artificial protein motor},

author = {Nathan J. Kuwada and Gerhard A. Blab and Heiner Linke},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77957750193&partnerID=40&md5=eb39d2cf0cdf264aa9c807cc40f7d15e},

doi = {10.1016/j.chemphys.2010.05.009},

year  = {2010},

date = {2010-01-01},

journal = {Chemical Physics},

volume = {375},

number = {2-3},

pages = {479-485},

note = {cited By 9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Bromley2009204,

title = {The tumbleweed: Towards a synthetic protein motor},

author = {Elizabeth H.C. Bromley and Nathan J. Kuwada and Martin J. Zuckermann and Roberta Donadini and Laleh Samii and Gerhard A. Blab and Greg J. Gemmen and Benjamin J. Lopez and Paul M.G. Curmi and Nancy R. Forde and Dek N. Woolfson and Heiner Linke},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-70350665969&partnerID=40&md5=8d9fbb9cf82eeef7e51b07fbbc509cf8},

doi = {10.2976/1.3111282},

year  = {2009},

date = {2009-01-01},

journal = {HFSP Journal},

volume = {3},

number = {3},

pages = {204-212},

note = {cited By 22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Chen20094701,

title = {Stretching submicron biomolecules with constant-force axial optical tweezers},

author = {Yi Fan Chen and Gerhard A. Blab and Jens Christian Meiners},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-68949114442&partnerID=40&md5=21b94c0763c8390f73ea62f7cdfcbcf7},

doi = {10.1016/j.bpj.2009.03.009},

year  = {2009},

date = {2009-01-01},

journal = {Biophysical Journal},

volume = {96},

number = {11},

pages = {4701-4708},

note = {cited By 39},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Lasne200714184,

title = {Label-free optical imaging of mitochondria in live cells},

author = {David Lasne and Gerhard A. Blab and Francesca A. de Giorgi and François Ichas and Brahim Lounis and Laurent Cognet},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-35349030875&partnerID=40&md5=630b4a8552ceebe01da4300cad109243},

doi = {10.1364/OE.15.014184},

year  = {2007},

date = {2007-01-01},

journal = {Optics Express},

volume = {15},

number = {21},

pages = {14184-14193},

note = {cited By 38},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Blab2006L13,

title = {Optical readout of gold nanoparticle-based DNA microarrays without silver enhancement},

author = {Gerhard A. Blab and Laurent Cognet and Stéphane Berciaud and Isabelle Alexandre and Dieter Husar and Josè A. Remacle and Brahim Lounis},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33646148710&partnerID=40&md5=f9cf30b08231721eec091e0c0245ea00},

doi = {10.1529/biophysj.105.076182},

year  = {2006},

date = {2006-01-01},

journal = {Biophysical Journal},

volume = {90},

number = {1},

pages = {L13-L15},

note = {cited By 38},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Berciaud2006b,

title = {Photothermal heterodyne imaging of individual metallic nanoparticles: Theory versus experiment},

author = {Stéphane Berciaud and David Lasne and Gerhard A. Blab and Laurent Cognet and Brahim Lounis},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33144468896&partnerID=40&md5=b635d9c34171870a3b7f93b62e725168},

doi = {10.1103/PhysRevB.73.045424},

year  = {2006},

date = {2006-01-01},

journal = {Physical Review B - Condensed Matter and Materials Physics},

volume = {73},

number = {4},

note = {cited By 112},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Lasne20064598,

title = {Single Nanoparticle Photothermal Tracking (SNaPT) of 5-nm gold beads in live cells},

author = {David Lasne and Gerhard A. Blab and Stéphane Berciaud and Martin Heine and Laurent Groc and Daniel Choquet and Laurent Cognet and Brahim Lounis},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33845383320&partnerID=40&md5=0183c24ef26a6d6a86b19f50f95e4c63},

doi = {10.1529/biophysj.106.089771},

year  = {2006},

date = {2006-01-01},

journal = {Biophysical Journal},

volume = {91},

number = {12},

pages = {4598-4604},

note = {cited By 153},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Blab2004495,

title = {Homogeneous Detection of Single Rolling Circle Replication Products},

author = {Gerhard A. Blab and Thomas Schmidt and Mats F. Nilsson},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0347128038&partnerID=40&md5=22d265eb0259549def245ec0d28c26a6},

doi = {10.1021/ac034987+},

year  = {2004},

date = {2004-01-01},

journal = {Analytical Chemistry},

volume = {76},

number = {2},

pages = {495-498},

note = {cited By 49},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Blab2004727,

title = {Simultaneous wide-field imaging and spectroscopy of localized fluorophores},

author = {Gerhard A. Blab and Silke Oellerich and Reinier Schumm and Thomas Schmidt},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-1942486849&partnerID=40&md5=0d179bab8c8b01a04ed877c9aca62b97},

doi = {10.1364/OL.29.000727},

year  = {2004},

date = {2004-01-01},

journal = {Optics Letters},

volume = {29},

number = {7},

pages = {727-729},

note = {cited By 9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Berciaud2004,

title = {Photothermal heterodyne imaging of individual nonfluorescent nanoclusters and nanocrystals},

author = {Stéphane Berciaud and Laurent Cognet and Gerhard A. Blab and Brahim Lounis},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-42749100048&partnerID=40&md5=881dcfe9b3232277b6df3d1281369128},

doi = {10.1103/PhysRevLett.93.257402},

year  = {2004},

date = {2004-01-01},

journal = {Physical Review Letters},

volume = {93},

number = {25},

note = {cited By 177},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Lommerse2004609,

title = {Single-Molecule Imaging of the H-Ras Membrane-Anchor Reveals Domains in the Cytoplasmic Leaflet of the Cell Membrane},

author = {Piet H.M. Lommerse and Gerhard A. Blab and Laurent Cognet and Gregory S. Harms and Bogusława E. Snaar-Jagalska and Herman P. Spaink and Thomas Schmidt},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346057912&partnerID=40&md5=ddeb5e2b0c00cb85ce1f95fce31a2d6b},

year  = {2004},

date = {2004-01-01},

journal = {Biophysical Journal},

volume = {86},

number = {1 I},

pages = {609-616},

note = {cited By 104},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Blab200171,

title = {Two-photon excitation action cross-sections of the autofluorescent proteins},

author = {Gerhard A. Blab and Piet H.M. Lommerse and Laurent Cognet and Gregory S. Harms and Thomas Schmidt},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0012776120&partnerID=40&md5=ddfc6b34fa8c01492e90625686dfe0a9},

doi = {10.1016/S0009-2614(01)01282-9},

year  = {2001},

date = {2001-01-01},

journal = {Chemical Physics Letters},

volume = {350},

number = {1-2},

pages = {71-77},

note = {cited By 92},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Harms20012639,

title = {Single-molecule imaging of L-type Ca2+ channels in live cells},

author = {Gregory S. Harms and Laurent Cognet and Piet H.M. Lommerse and Gerhard A. Blab and Heike Kahn and Roland Gamsjäger and Herman P. Spaink and Nikolai M. Soldatov and Christoph Romanin and Thomas Schmidt},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034759494&partnerID=40&md5=fca270cf07f3aa023b7209793ef796e9},

year  = {2001},

date = {2001-01-01},

journal = {Biophysical Journal},

volume = {81},

number = {5},

pages = {2639-2646},

note = {cited By 133},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Harms20012396,

title = {Autofluorescent proteins in single-molecule research: Applications to live cell imaging microscopy},

author = {Gregory S. Harms and Laurent Cognet and Piet H.M. Lommerse and Gerhard A. Blab and Thomas Schmidt},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035033890&partnerID=40&md5=5655a2d31fd7439fb8a75238144f70e4},

year  = {2001},

date = {2001-01-01},

journal = {Biophysical Journal},

volume = {80},

number = {5},

pages = {2396-2408},

note = {cited By 169},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Cognet20004052,

title = {Simultaneous dual-color and dual-polarization imaging of single molecules},

author = {Laurent Cognet and Gregory S. Harms and Gerhard A. Blab and Piet H.M. Lommerse and Thomas Schmidt},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0001759698&partnerID=40&md5=55e9630e8cbae934c79a89d9470a24a2},

year  = {2000},

date = {2000-01-01},

journal = {Applied Physics Letters},

volume = {77},

number = {24},

pages = {4052-4054},

note = {cited By 48},

keywords = {},

pubstate = {published},

tppubtype = {article}

}