夏呈辉 (Chénghuī Xià)

Doctoral Candidate, Single quantum dot imaging, fabrication of a quantum-dot based FRET system.
Ornstein Lab. 119
tel: +31 30 253 2286

c.xia@uu.nl

Chenghui has started his combined master’s and doctoral studies at the 中国海洋大学 (Ocean University of China) in Qingdao in 2013. In 2014, he was awarded a scholarship by the China Scholarship Council (CSC)  and now pursues a doctoral degree at Utrecht University under the supervision of Profs. Hans Gerritsen, Andries Meijerink and Celso de Mello Donegá.

Research :

Dark quenchers, namely non-fluorescent dyes, have been commonly applied in Förster resonance energy transfer (FRET) based fluorogenic probes for protease activity detection, nucleic acid hybridization and real-time polymerase chain reaction. In FRET-based systems, a specific quencher is normally able to quench the fluorescence only from those fluorophore donors that have significant overlap of their emission spectra with the absorption spectrum of the quencher, and when the donor and quencher are brought into close proximity (much less than 10 nm).

In my project, we will fabricate a FRET system using CuInS2/ZnS QDs as donor, while a dark dye as an acceptor efficiently quenches fluorescence from a wide range of donors, spanning from 500 to 800 nm emission. High quality water-soluble CuInS2/ZnS QDs, with photoluminescence quantum yield (PLQY) around 30%, were obtained via ligand exchange. Furthermore, the emission spectra of these water-soluble QDs can be tuned by controlling the size of their cores. By tuning the PLQY of donor and the degree of spectral overlap between the donor emission and the acceptor absorption, different Förster distance (R0) and efficiency of energy transfer (E) can be obtained. These optimized results will contribute to the further investigation on the lipo-protein biointeractions via FRET.

Publications

2018

Xia, Cheng-Hui; Winckelmans, N.; Prins, P. T.; Bals, S.; Gerritsen, Hans C.; Donega, Celso De Mello

Near-Infrared-Emitting CuInS2/ZnS Dot-in-Rod Colloidal Heteronanorods by Seeded Growth Journal Article

In: Journal of the American Chemical Society, vol. 140, no. 17, pp. 5755-5763, 2018, (cited By 1).

Abstract | Links | BibTeX

Xia, Cheng-Hui; Wu, W.; Yu, T.; Xie, X.; Oversteeg, C. V.; Gerritsen, Hans C.; Donega, Celso De Mello

Size-Dependent Band-Gap and Molar Absorption Coefficients of Colloidal CuInS2 Quantum Dots Journal Article

In: ACS Nano, 2018, (cited By 0; Article in Press).

Abstract | Links | BibTeX

2017

Xia, Cheng-Hui; Meeldijk, Johannes D.; Gerritsen, Hans C.; de Mello-Donega, Celso

Highly Luminescent Water-Dispersible NIR-Emitting Wurtzite CuInS2/ZnS Core/Shell Colloidal Quantum Dots Journal Article

In: Chemistry of Materials, vol. 29, no. 11, pp. 4940-4951, 2017.

Abstract | Links | BibTeX

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