TRAK/Milton Motor-Adaptor Proteins Steer Mitochondrial Trafficking to Axons and Dendrites

Myrrhe van Spronsen, Marina Mikhaylova, Joanna Lipka, Max A. Schlager, Dave J. van den Heuvel, Marijn Kuijpers, Phebe S. Wulf, Nanda Keijzer, Jeroen A.A. Demmers, Lukas C. Kapitein, Dick Jaarsma, Hans C. Gerritsen, Anna S. Akhmanova, Casper C. Hoogenraad: TRAK/Milton Motor-Adaptor Proteins Steer Mitochondrial Trafficking to Axons and Dendrites. In: Neuron, vol. 77, no. 3, pp. 485-502, 2013, (cited By (since 1996) 0).

Abstract

In neurons, the distinct molecular composition of axons and dendrites is established through polarized targeting mechanisms, but it is currently unclear how nonpolarized cargoes, such as mitochondria, become uniformly distributed over these specialized neuronal compartments. Here, we show that TRAK family adaptor proteins, TRAK1 and TRAK2, which link mitochondria to microtubule-based motors, are required for axonal and dendritic mitochondrial motility and utilize different transport machineries to steer mitochondria into axons and dendrites. TRAK1 binds to both kinesin-1 and dynein/dynactin, is prominently localized in axons, and is needed for normal axon outgrowth, whereas TRAK2 predominantly interacts with dynein/dynactin, is more abundantly present in dendrites, and is required for dendritic development. These functional differences follow from their distinct conformations: TRAK2 preferentially adopts a head-to-tail interaction, which interferes with kinesin-1 binding and axonal transport. Our study demonstrates how the molecular interplay between bidirectional adaptor proteins and distinct microtubule-based motors drives polarized mitochondrial transport. van Spronsen et al. show that mitochondria utilize different machineries to steer their transport into axons and dendrites. The molecular interplay between mitochondrial adaptor protein family TRAK/Milton and distinct microtubule-based motors drives polarized mitochondrial transport. © 2013 Elsevier Inc.

BibTeX (Download)

@article{vanSpronsen2013485,
title = {TRAK/Milton Motor-Adaptor Proteins Steer Mitochondrial Trafficking to Axons and Dendrites},
author = {Myrrhe van Spronsen and Marina Mikhaylova and Joanna Lipka and Max A. Schlager and Dave J. van den Heuvel and Marijn Kuijpers and Phebe S. Wulf and Nanda Keijzer and Jeroen A.A. Demmers and Lukas C. Kapitein and Dick Jaarsma and Hans C. Gerritsen and Anna S. Akhmanova and Casper C. Hoogenraad},
url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84873279659&partnerID=40&md5=53f917e82bb41c31d7cd3eaf1ebdb2b4},
year  = {2013},
date = {2013-01-01},
journal = {Neuron},
volume = {77},
number = {3},
pages = {485-502},
abstract = {In neurons, the distinct molecular composition of axons and dendrites is established through polarized targeting mechanisms, but it is currently unclear how nonpolarized cargoes, such as mitochondria, become uniformly distributed over these specialized neuronal compartments. Here, we show that TRAK family adaptor proteins, TRAK1 and TRAK2, which link mitochondria to microtubule-based motors, are required for axonal and dendritic mitochondrial motility and utilize different transport machineries to steer mitochondria into axons and dendrites. TRAK1 binds to both kinesin-1 and dynein/dynactin, is prominently localized in axons, and is needed for normal axon outgrowth, whereas TRAK2 predominantly interacts with dynein/dynactin, is more abundantly present in dendrites, and is required for dendritic development. These functional differences follow from their distinct conformations: TRAK2 preferentially adopts a head-to-tail interaction, which interferes with kinesin-1 binding and axonal transport. Our study demonstrates how the molecular interplay between bidirectional adaptor proteins and distinct microtubule-based motors drives polarized mitochondrial transport. van Spronsen et al. show that mitochondria utilize different machineries to steer their transport into axons and dendrites. The molecular interplay between mitochondrial adaptor protein family TRAK/Milton and distinct microtubule-based motors drives polarized mitochondrial transport. © 2013 Elsevier Inc.},
note = {cited By (since 1996) 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}